|   别称

 

IL1-B; IL1-Beta; IL1F2; IL-1β; Interleukin-1 Family Member 2; Catabolin

 

 

|   IL-1β 简介

 

白细胞介素1β(IL-1β)是一种在各种细胞中产生的促炎症细胞因子,包括单核细胞、组织巨噬细胞、角质细胞和其他上皮细胞。IL-1α和IL-1β都与相同的受体结合,并具有类似甚至相同的生物学特性。这些细胞因子具有广泛的活性,包括通过诱导IL-2的释放刺激胸腺细胞的增殖,B细胞的成熟和增殖,有丝分裂的FGF样活性和刺激滑膜细胞释放前列腺素和胶原酶的能力。然而,IL-1β是一种分泌型的细胞因子,而IL-1α主要是一种细胞相关的细胞因子。

 

 

|   特异性

 

可检测样本中人的IL-1β,且与其类似物无明显交叉反应

 

 

|   典型数据及参考曲线

 

由于实验操作条件的不同( 如操作者、移液技术、洗板技术和稳定条件等),标准曲线的OD值会有所差异。以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。

 

浓度(pg/mL) 80 40 20 10 5 2.5 1.25 0
OD值 2.51 1.96 1.24 0.72 0.46 0.3 0.22 0.09
校正OD值 2.42 1.87 1.15 0.63 0.37 0.21 0.13 -

 

注意:本图仅供参考,应以同次试验标准品所绘标准曲线计算标本含量。

 

 

|   重复性

 

板内变异系数小于10%,板间变异系数小于10%

 

 

|   回收率

 

在选取的健康人血清、血浆、细胞培养上清中加入3个不同浓度水平的人IL-1β,计算回收率

 

样本类型 范围 平均回收率
血清(n=8) 83-100 94
血浆(n=8) 92-101 97
细胞培养上清(n=8) 97-105 102

 

 

|   线性稀释

 

分别在选取的4份健康人血清、血浆、细胞培养上清中加入高浓度人IL-1β,在标准曲线动力学范围内进行稀释,评估线性。

 

稀释比例 回收率(%) 血清 血浆 细胞培养上清
1:2 范围(%) 84-95 88-96 92-111
平均回收率(%) 91 94 96
1:4 范围(%) 85-102 87-108 101-115
平均回收率(%) 94 98 106

 

 

注:ELISA试剂盒检测范围等数据,因检测样本 的不同而调整,实际数据以随货说明书为准。

 

 

|   ELISA操作视频

 

ELISA操作视频        ELISA保存温度

 

 

|   ELISA操作前必读 / 下载

 

样本处理    血液样本    加样    温育

 

洗板    拟合曲线    常见问题    下载

 

 

|   客户发表的文献

 

 

Interaction with CD68 and regulation of GAS6 expression by endosialin in fibroblasts drives recruitment and polarization of macrophages in hepatocellular carcinoma

Rank: IF 11.2

Journal: Cancer Research

Year: 2020

DOI: 10.1158/0008-5472.CAN-19-2691

 


 

5-Bromo-3,4-dihydroxybenzaldehyde attenuates endothelial cells injury from high glucose-induced damage

Rank: IF 7.5

Journal: Biomedicine & Pharmacotherapy

Year: 2022

DOI: 10.1016/j.biopha.2022.113793

 


 

The Effect of Allograft Inflammatory Factor-1 on Inflammation, Oxidative Stress, and Autophagy via miR-34a/ATG4B Pathway in Diabetic Kidney Disease

Rank: IF 7.31

Journal: Oxidative Medicine and Cellular Longevity

Year: 2022

DOI: 10.1155/2022/1668000

 


 

Synthesis and pharmacological validation of fluorescent diarylsulfonylurea analogues as NLRP3 inhibitors and imaging probes

Rank: IF 6.7

Journal: European Journal of Medicinal Chemistry

Year: 2022

DOI: 10.1016/j.ejmech.2022.114338

 


 

Pyroptosis is involved in the inhibitory effect of FL118 on growth and metastasis in colorectal cancer

Rank: IF 6.1

Journal: Life Sciences

Year: 2020

DOI: 10.1016/j.lfs.2020.118065

 


 

The expression of parthanatos markers and miR-7 mimic protects photoreceptors from parthanatos by repressing α-synuclein in retinal detachment

Rank: IF 6

Journal: American Journal of Pathology

Year: 2023

DOI: 10.1016/j.ajpath.2023.06.011

 


 

Anti-Inflammatory Effect of Geniposide on Regulating the Functions of Rheumatoid Arthritis Synovial Fibroblasts via Inhibiting Sphingosine-1-Phosphate Receptors1/3 Coupling Gαi/Gαs Conversion

Rank: IF 5.6

Journal: Frontiers in Pharmacology

Year: 2020

DOI: 10.3389/fphar.2020.584176

 


 

The rCC16 Protein Protects Against LPS-Induced Cell Apoptosis and Inflammatory Responses in Human Lung Pneumocytes

Rank: IF 5.6

Journal: Frontiers in Pharmacology

Year: 2020

DOI: 10.3389/fphar.2020.01060

 


 

PINK1-Dependent Mitophagy Reduced Endothelial Hyperpermeability and Cell Migration Capacity Under Simulated Microgravity

Rank: IF 5.5

Journal: Frontiers in Cell and Developmental Biology

Year: 2022

DOI: 10.3389/fcell.2022.896014

 


 

Anti‑β2GPI/β2GPI induces neutrophil pyroptosis and thereby enhances ICAM‑1 and IL‑8 expression in endothelial cells

Rank: IF 5.4

Journal: International Journal of Molecular Medicine

Year: 2022

DOI: 10.3892/ijmm.2022.5120