中文名称：丝/苏氨酸蛋白质激酶II α抗体

英文名称：CK II alpha

别    名：STK; Casein kinase II Alpha; CKII alpha; Serine/Threonine Protein Kinase II Alpha; CKⅡalpha; Casein kinase 2 alpha 1 polypeptide; Casein kinase II alpha 1 subunit; Casein kinase II alpha chain; Casein kinase II alpha subunit; Casein kinase II subunit alpha; Casein kinase II alpha 1; CK II alpha;  CKIIalpha; CSK21_HUMAN; CK II; CK2 alpha; CK2 catalytic subunit alpha; CK2A1; CKII; CSNK2A1.

产品类型：

研究领域：细胞生物  信号转导  细胞凋亡  激酶和磷酸酶  

抗体来源：Rabbit

克隆类型：Polyclonal

克 隆 号：

交叉反应：Human, Mouse, Rat, Dog, Cow, 

浓    度：1mg/1ml

分 子 量：45kDa

细胞定位：

储 存 液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce

纯化方法：affinity purified by Protein A

免 疫 原：KLH conjugated synthetic peptide derived from human CK II al

性    状：Lyophilized or Liquid

亚    型：IgG

PubMed：PubMed

产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）

 

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

保存条件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

产品介绍:background:Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. While this gene is found on chromosome 20, a related transcribed pseudogene is found on chromosome 11. Three transcript variants encoding two different proteins have been found for this gene. [provided by RefSeq, Jul 2008]

 

Function:Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Regulates numerous cellular processes, such as cell cycle progression, apoptosis and transcription, as well as viral infection. May act as a regulatory node which integrates and coordinates numerous signals leading to an appropriate cellular response. During mitosis, functions as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage. Also required for p53/TP53-mediated apoptosis, phosphorylating 'Ser-392' of p53/TP53 following UV irradiation. Can also negatively regulate apoptosis. Phosphorylates the caspases CASP9 and CASP2 and the apoptotic regulator NOL3. Phosphorylation protects CASP9 from cleavage and activation by CASP8, and inhibits the dimerization of CASP2 and activation of CASP8. Regulates transcription by direct phosphorylation of RNA polymerases I, II, III and IV. Also phosphorylates and regulates numerous transcription factors including NF-kappa-B, STAT1, CREB1, IRF1, IRF2, ATF1, SRF, MAX, JUN, FOS, MYC and MYB. Phosphorylates Hsp90 and its co-chaperones FKBP4 and CDC37, which is essential for chaperone function. Regulates Wnt signaling by phosphorylating CTNNB1 and the transcription factor LEF1. Acts as an ectokinase that phosphorylates several extracellular proteins. During viral infection, phosphorylates various proteins involved in the viral life cycles of EBV, HSV, HBV, HCV, HIV, CMV and HPV. Phosphorylates PML at 'Ser-565' and primes it for ubiquitin-mediated degradation. 

 

Subunit:Heterotetramer composed of two catalytic subunits (alpha chain and/or alpha' chain) and two regulatory subunits (beta chains). The tetramer can exist as a combination of 2 alpha/2 beta, 2 alpha'/2 beta or 1 alpha/1 alpha'/2 beta subunits. Also part of a CK2-SPT16-SSRP1 complex composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B, which forms following UV irradiation. Interacts with RNPS1. Interacts with SNAI1. Interacts with PML (isoform PML-12).buy viagra online viagra for sale uk common side effects viagra

 

Post-translational modifications:Phosphorylated at Thr-344, Thr-360, Ser-362 and Ser-370 by CDK1 in prophase and metaphase and dephosphorylated during anaphase. Phosphorylation does not directly affect casein kinase 2 activity, but may contribute to its regulation by forming binding sites for interacting proteins and/or targeting it to different compartments.

 

Similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.Contains 1 protein kinase domain. 

 

Gene ID:1457

 

Database links:

Entrez Gene: 1457 Human

 

 

Entrez Gene: 432370 Chicken

 

 

Entrez Gene: 282419 Cow

 

 

Entrez Gene: 12995 Mouse

 

 

Entrez Gene: 116549 Rat

 

 

Omim: 115440 Human

 

 

SwissProt: P21868 Chicken

 

 

SwissProt: P68399 Cow

 

 

SwissProt: P68400 Human

 

 

SwissProt: Q60737 Mouse

 

 

SwissProt: P19139 Rat

 

 

Unigene: 644056 Human

 

 

Unigene: 654675 Human

 

 

Unigene: 23692 Mouse

 

 

Unigene: 4231 Rat

 

 

Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

STKII参与调节细胞周期阻滞、细胞生长、细胞凋亡.在细胞形态学和动力学以及细胞转化的调控方面起到了很重要的作用。